Movies

This movie displays an FM4-64 labeled embryo showing the first few cell divisions.

This movie displays an FM4-64 labeled embryo showing the first few cell divisions.

This movie displays an FM4-64 labeled embryo showing the first few cell divisions.

Movie of developing parhyale embyro, 3.5 - 10 Days (20.6 MB). Starting at early limb development up to near hatching.

Movie of developing parhyale embyro, 3.5 - 10 Days (20.6 MB). Starting at early limb development up to near hatching.

Movie of developing parhyale embyro, 3.5 - 10 Days (20.6 MB). Starting at early limb development up to near hatching.

This is a movie of the migration and divisions of the Mesoteloblasts, the mesodermal stem cells. The Mesoteloblasts are the eight large cells migrating from right to left in the video. As they migrate, they undergo a series of asymmetric divisions to produce the mesoblasts. One row of mesoblasts will give rise to one segment's worth of mesoderm. The embryo is orientated with anterior to the right, posterior to the left, and ventral towards the viewer. To create this movie, DsRed-NLS mRNA (which the embryo translated into a fluorescent protein) was injected into the left and right segmental mesoderm precursor cells at the eight-cell stage (7hrs of development). Filming was begun at approximately three days of development and lasts for about one day. This movie also illustrates the dangers of working in California. On July 20, 2007 at 4:42 am, an earthquake with a magnitude of 4.2 took place in the Bay Area. This caused the embryo in this film to move. Instead of being orientated on its back with the head and mid-body region in view, the embryo is now orientated on its head so that only the tail region is seen. The real-time stamp in the upper right corner of the video confirms that this movement took place at the same time as the earthquake.

This is a movie of the migration and divisions of the Mesoteloblasts, the mesodermal stem cells. The Mesoteloblasts are the eight large cells migrating from right to left in the video. As they migrate, they undergo a series of asymmetric divisions to produce the mesoblasts. One row of mesoblasts will give rise to one segment's worth of mesoderm. The embryo is orientated with anterior to the right, posterior to the left, and ventral towards the viewer. To create this movie, DsRed-NLS mRNA (which the embryo translated into a fluorescent protein) was injected into the left and right segmental mesoderm precursor cells at the eight-cell stage (7hrs of development). Filming was begun at approximately three days of development and lasts for about one day. This movie also illustrates the dangers of working in California. On July 20, 2007 at 4:42 am, an earthquake with a magnitude of 4.2 took place in the Bay Area. This caused the embryo in this film to move. Instead of being orientated on its back with the head and mid-body region in view, the embryo is now orientated on its head so that only the tail region is seen. The real-time stamp in the upper right corner of the video confirms that this movement took place at the same time as the earthquake.

This is a movie of the migration and divisions of the Mesoteloblasts, the mesodermal stem cells. The Mesoteloblasts are the eight large cells migrating from right to left in the video. As they migrate, they undergo a series of asymmetric divisions to produce the mesoblasts. One row of mesoblasts will give rise to one segment's worth of mesoderm. The embryo is orientated with anterior to the right, posterior to the left, and ventral towards the viewer. To create this movie, DsRed-NLS mRNA (which the embryo translated into a fluorescent protein) was injected into the left and right segmental mesoderm precursor cells at the eight-cell stage (7hrs of development). Filming was begun at approximately three days of development and lasts for about one day. This movie also illustrates the dangers of working in California. On July 20, 2007 at 4:42 am, an earthquake with a magnitude of 4.2 took place in the Bay Area. This caused the embryo in this film to move. Instead of being orientated on its back with the head and mid-body region in view, the embryo is now orientated on its head so that only the tail region is seen. The real-time stamp in the upper right corner of the video confirms that this movement took place at the same time as the earthquake.

Expression of hedgehog (hh) and odd-paired 1 (opa1) in the posterior ectoderm of Parhyale hawaiensis. (Ventral view, anterior is up and posterior is down.) Expression of hh begins in PSPR cells approximately 1 hour prior to their division. After a PSPR divides, hh expression is maintained in a/b cells and lost in c/d cells. After an a/b cell divides, hh expression is maintained in a cells and lost in b cells. opa1 is first expressed in c/d cells approximately 1 hour after they are formed. After a c/d cell divides, opa1 is expressed in both c and d cells. Expression is eventually lost in the most medial c and d cells.

Expression of hedgehog (hh) and odd-paired 1 (opa1) in the posterior ectoderm of Parhyale hawaiensis. (Ventral view, anterior is up and posterior is down.) Expression of hh begins in PSPR cells approximately 1 hour prior to their division. After a PSPR divides, hh expression is maintained in a/b cells and lost in c/d cells. After an a/b cell divides, hh expression is maintained in a cells and lost in b cells. opa1 is first expressed in c/d cells approximately 1 hour after they are formed. After a c/d cell divides, opa1 is expressed in both c and d cells. Expression is eventually lost in the most medial c and d cells.

The posterior ectoderm of Parhyale hawaiensis has a stereotyped cell lineage pattern. (Ventral view, anterior is up and posterior is down.) A loosely organized group of cells in the posterior condenses to form ordered rows termed Parasegmental Precursor Rows (PSPRs). These PSPR rows undergo two mediolateral waves of division; the first wave forms rows a/b and c/d, and the second waves forms rows a, b, c, and d. Rows a, b, c, and d comprise a single parasegment, which is a genealogical unit that specifies segmental boundaries later in development.

The posterior ectoderm of Parhyale hawaiensis has a stereotyped cell lineage pattern. (Ventral view, anterior is up and posterior is down.) A loosely organized group of cells in the posterior condenses to form ordered rows termed Parasegmental Precursor Rows (PSPRs). These PSPR rows undergo two mediolateral waves of division; the first wave forms rows a/b and c/d, and the second waves forms rows a, b, c, and d. Rows a, b, c, and d comprise a single parasegment, which is a genealogical unit that specifies segmental boundaries later in development.

Expression of hedgehog (hh) and odd-paired 1 (opa1) in the posterior ectoderm of Parhyale hawaiensis. (Ventral view, anterior is up and posterior is down.) Expression of hh begins in PSPR cells approximately 1 hour prior to their division. After a PSPR divides, hh expression is maintained in a/b cells and lost in c/d cells. After an a/b cell divides, hh expression is maintained in a cells and lost in b cells. opa1 is first expressed in c/d cells approximately 1 hour after they are formed. After a c/d cell divides, opa1 is expressed in both c and d cells. Expression is eventually lost in the most medial c and d cells.

The posterior ectoderm of Parhyale hawaiensis has a stereotyped cell lineage pattern. (Ventral view, anterior is up and posterior is down.) A loosely organized group of cells in the posterior condenses to form ordered rows termed Parasegmental Precursor Rows (PSPRs). These PSPR rows undergo two mediolateral waves of division; the first wave forms rows a/b and c/d, and the second waves forms rows a, b, c, and d. Rows a, b, c, and d comprise a single parasegment, which is a genealogical unit that specifies segmental boundaries later in development.